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. 2010 Sep 1;5(9):e12515. doi: 10.1371/journal.pone.0012515

Figure 2. Lodamin inhibits angiogenesis, inflammation and edema in the DTH reaction.

Figure 2

(A) Lodamin suppressed ear swelling in DTH reactions elicited by oxazolone, photos of representative ears of Lodamin treated or untreated mice are circled. Bar = 1 cm (B) Ear swelling is represented as change in thickness (in µm) compared to original ear thickness. Mice treated with Lodamin (white squares) showed a significant reduction in ear thickness compared to control mice (black squares). The differences were statistically significant from day 2 to day 12 post challenge, excluding day 5 (P<0.05). Data are presented as an average ±SEM, n = 5. (C) Immunohistological analysis of mouse ears post DTH reaction: H&E staining (a–f) and specific staining of endothelial cell marker CD-31 (g–h). In H&H staining, control ears exhibited an excessive inflammation and edema (a–d), including fibroblast proliferation (marked as F) and spongiosis pointed by arrows compared with Lodamin treated mice (e–f). Bar = 50µm.