Figure 6. Retinal ERG and protein analysis post Lodamin treatments.

Effects of intravitreal Lodamin on retinal function. (A) Representative full-field flash ERG responses from the eyes of mice 14 days after intra-ocular injection of vehicle (left) or 300µg /eye Lodamin (right). (B) Representative responses to 8 Hz flicker (C) The 5^th to 95^th prediction limits for ERG parameters in vehicle treated rats are shown; the dashed line represents the normal mean of all the paramters. Symbols are parameters values of individual Lodamin-treated mice, and represent the same mouse in each column. Data are ΔLogNormal (eq. 3). Only the saturating amplitude of the rod photoresponse (Rm _P3) differed significantly after Lodamin. (D) Effect of oral Lodamin treatment on protein levels of pro-angiogenic and pro-inflammatory factors. (D) Levels of MCP-1 and VEGF extracted from whole eye tissues from naïve mice, mice treated with Lodamin (30mg/kg, oral every day for 7 d) or vehicle (same equivalent amount of mPEG-PLA micelles). Data of ELISA assay are presented as a mean of pg/ml concentration ± SD (n = 6, * P<0.05, t-test). Bottom: ELISA quantification of secreted MCP-1 from RPE to the medium post 24 h incubation with Lodamin at different doses (0.1–100 nM TNP-470 equivalent). (E) Western blot protein analysis of samples of tissue lysis of retina or choroid of the different treatment groups: N = naïve mice, V = vehicle treated mice, L = Lodamin treated mice. Factors detected are TNFα (26 kDa) (F) Zymogram showing MMP-2 and MMP-9 activity in ocular tissue following oral Lodamin treatment of mice (30 mg/kg/day 7 days).