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. 2010 Sep 8;99(5):1620–1627. doi: 10.1016/j.bpj.2010.06.039

Figure 1.

Figure 1

The modal unfolding force, F∗, as a function of the loading rate, RF∗, calculated at each F∗. The pulling velocities at which the F∗ were determined are indicated (top of the graph). The loading rates, RF∗, were calculated by modeling the polymeric spacer as a wormlike chain (see Methods for details). (Error bars) Bin-width used to construct the unfolding force histograms. (Dashed lines) Piecewise fits of the data at 200–4000 nm/s (dark dashed line/red online) and at 1–20 nm/s (light dashed line/green online) to (kBT/xin/out)ln(RF∗) + Cin/out), respectively. The force-dependencies are xF∗in = 4.6 Å and xF∗out = 21.6 Å for the high- and low-velocity regimes, respectively. (Continuous line/red online) Fit to the kinetic scheme shown at the bottom of Fig. 5.