Figure 2.

Effect of dilution of oleate micelles toward neutrality on CF leakage of 200 nm DOPC seed vesicles. Time point 1 shows the relative fluorescence intensity of a dispersion of DOPC vesicles loaded with CF at a self-quenching concentration. At time point 2, an excess of oleate pH 10.5 was added, such that the final number of chains was 2.5 times greater than the number before oleate addition. At time point 3 the vesicles were lysed by 10% TX-100. The signal drop to zero occurred when the cuvette was removed from the spectrofluorometer for the addition of oleate or TX-100.