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Michaelis-Menten analysis of inhibition, showing the initial reaction velocity versus substrate (2,4,6-TCP) concentration. Assays were conducted on WT-DHP without the presence of inhibitor, WT-DHP with 125 μM 4-BP, WT-DHP with 250 μM 4-BP, and WT-DHP with 500 μM 4-BP. The Michaelis-Menten fit parameters are given in Table 2.
