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. 2010 Sep 8;99(5):1539–1545. doi: 10.1016/j.bpj.2010.06.059

Figure 2.

Figure 2

(A) Membrane rigidity, κΛ=0, as a function of Sar1p-GMPPNP concentration. The blurred box is drawn at a concentration at which spontaneous membrane vesiculation is observed, indicating near-zero rigidity. (Circles and triangles) Data from experiments at 1× (180 mM) and 0.05× (9 mM) HKM buffer, respectively. (Error bars) Standard errors of the means. (B) Spontaneous membrane disintegration. Fluorescence images of a region of membrane before (left) and after (right) the addition of Sar1p-GMPPNP at 400 μg/mL. (Bar) 10 μm. See also Movie S1, provided as Supporting Material.