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. 2010 Sep 8;99(5):1539–1545. doi: 10.1016/j.bpj.2010.06.059

Figure 3.

Figure 3

(A) The product of the tether retraction force (f) and radius (R) plotted as a function of R, for 80 μg/mL Δ23-Sar1-GMPPNP, which lacks the amphipathic α-helix of wild-type Sar1. A positive slope indicates a negative coupling between the protein and the local curvature, or equivalently a concave effective spontaneous curvature. (B) fR versus R for 80 μg/mL Sar1p-GMPPNP, and (C) fR versus R for membranes with no Sar1, with the same plot axes as panel A. The data plotted are from experiments in 0.05× HKM buffer. (Solid lines) Linear fits.