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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Insect Biochem Mol Biol. 2010 Apr 21;40(6):487–495. doi: 10.1016/j.ibmb.2010.04.008

Fig. 3. Isolation of M. sexta PGRP1 from baculovirus-infected Sf9 cells.

Fig. 3

Left panel, Coomassie blue staining; central panel, immunoblot analysis using anti-(His)5 as the first antibody; right panel, immunoblot analysis using diluted PGRP1 antiserum as the first antibody. Conditioned cell culture medium (lane 1, 10 μl), proteins eluted from dextran sulfate-Sepharose (lane 2, 10 μl), and affinity-purified PGRP1 from Ni2+-NTA agarose (lane 3, 10 μl) were separated by 15% SDS-PAGE, along with pre-stained molecular weight standards (M) with their sizes indicated on the left.