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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Insect Biochem Mol Biol. 2010 Apr 21;40(6):487–495. doi: 10.1016/j.ibmb.2010.04.008

Fig. 5. Binding of M. sexta PGRP1 to insoluble PGs (A, C, E, G) or cells (B, D, F, H) of different bacteria.

Fig. 5

As described in Sections 2.8 and 2.9, binding assays were performed using the purified PGRP1 and PGs or whole bacteria. The total, unbound, and bound fractions were separated by SDS-PAGE followed by immunoblot analysis using anti-(His)5 as the first antibody.