Skip to main content
. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Insect Biochem Mol Biol. 2010 Apr 21;40(6):487–495. doi: 10.1016/j.ibmb.2010.04.008

Fig. 7. Enhancement of proPO activation in plasma from naïve larvae by peptidoglacans in the absence or presence of M. sexta PGRP1.

Fig. 7

As described in Section 2.10, PGs of M. luteus (A), S. aureus (B), S. aureus (C, soluble), B. megaterium (D), B. subtilis (E), and E. coli (F, soluble) were separately incubated with plasma and purified PGRP1 for 60 min at room temperature. PO activity (#4) was measured and plotted as mean ± SEM (n = 3), along with those of the controls (#1, plasma only; #2, plasma and PG; #3, plasma and PGRP1). Since interaction of plasma factors with elicitor (#2 - #1) and co-presence of plasma and exogenous PGRP1 (#3 - #1) both lead to proPO activation, an interaction of elicitor and exogenous PGRP1 in plasma (#4 - #1) is expected to increase proPO activation to a level significantly higher than the sum of the two components [i.e. (#2 - #1) + (#3 - #1)]. To detect a possible synergistic effect of elicitor-PGRP1 interaction, the PO activity changes represented by (#4 - #1) and (#2 + #3 – 2×#1) are compared using unpaired t-test. An asterisk (*) on #4 indicates that (#4 - #1) is significantly higher (p < 0.05).