Table 1.
Hematopoietic and endothelial differentiation of human induced pluripotent and embryonic stem cells*
| Human induced pluripotent stem cells | Human embryonic stem cells | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Foreskin-1 | Foreskin-2 | IMR90-1 | IMR90-4 | SK46 M3-6 | SK46 M4-8 | SK46 M4-10 | H1 | H7 | H9 | H13 | H14 | |
| Human cells (TRA-1-85+7-AAD−), ×105 | 86.8 ± 19.3 | 68.5 ± 8.8 | 78.5 ± 6.7 | 84.3 ± 13.7 | 97.5 ± 13.1 | 60.2 ± 8.2 | 96.6 ± 9.6 | 130.4 ± 10.2 | 101.7 ± 16.3 | 121.3 ± 19.8 | 106.5 ± 18.3 | 86.0 ± 11.4 |
| CD31+CD43− Endothelial cells, ×105 (%within gated human cells) |
4.0 ± 0.9 (5.2 ± 0.3) |
4.1 ± 0.5 (6.0 ± 0.2) |
2.7 ± 0.3 (3.4 ± 0.3) |
2.4 ± 0.5 (2.8 ± 0.4) |
4.5 ± 0.5 (4.7 ± 0.4) |
2.1 ± 0.5 (3.5 ± 1.1) |
0.37 ± 0.6 (4.2 ± 0.2) |
7.2 ± 0.6 (5.3 ± 0.4) |
5.3 ± 0.9 (5.5 ± 0.9) |
5.6 ± 1.6 (4.3 ± 1.5) |
4.6 ± 0.6 (4.6 ± 0.6) |
4.2 ± 0.9 (4.7 ± 0.5) |
| CD43+ Hematopoietic cells, ×105 (%within gated human cells) |
11.8 ± 3.0 (16.1 ± 2.7) |
3.8 ± 1.1 (5.7 ± 1.7) |
6.7 ± 1.4 (8.7 ± 1.5) |
1.5 ± 0.1 (1.9 ± 0.3) |
4.2 ± 0.7 (4.2 ± 0.2) |
1.8 ± 0.7 (3.1 ± 1.4) |
9.4 ± 1.6 (11.5 ± 1.9) |
12.2 ± 3.0 (9.6 ± 1.9) |
9.7 ± 3.0 (9.6 ± 2.5) |
6.3 ± 1.7 (5.1 ± 2.2) |
3.0 ± 0.9 (3.1 ± 1.0) |
16.8 ± 5.2 (18.1 ± 4.1) |
| CD41a+CD235a+CD45− cells, ×105 (% within gated CD43+cells) |
9.9 ± 2.7 (69.5 ± 6.1) |
3.2 ± 1.0 (84.7 ± 2.0) |
5.5 ± 1.7 (76.0 ± 5.0) |
1.3 ± 0.1 (81.7 ± 0.8) |
2.8 ± 0.4 (67.4 ± 3.8) |
1.3 ± 0.5 (73.2 ± 4.9) |
6.4 ± 1.4 (66.9 ± 2.3) |
9.72 ± 3.4 (72.4 ± 4.9) |
8.7 ± 2.9 (83.4 ± 2.5) |
4.8 ± 1.2 (77.4±7.1) |
2.3 ± 0.6 (79.4 ± 3.5) |
14.9 ± 5.9 (87.0 ± 3.6) |
| CD41a−CD235a−CD45− cells, ×105 (%within gated CD43+cells) |
1.4 ± 0.4 (9.8 ± 0.9) |
0.2 ± 0.0 (6.9 ± 0.8) |
0.4 ± 0.1 (5.8 ± 0.5) |
< 0.1 (5.6 ± 1.0) |
0.8 ± 0.2 (17.6 ± 2.0) |
0.1 ± 0.0 (5.7 ± 0.5) |
1.0 ± 0.2 (11.0 ± 0.7) |
1.2 ± 0.1 (11.7 ± 2.8) |
0.6 ± 0.2 (5.5 ± 0.5) |
0.8 ± 0.3 (10.4± 3.3) |
0.2 ± 0.1 (6.2 ± 3.5) |
0.8 ± 0.3 (4.9 ± 0.7) |
| CD41a−CD235a−CD45+ cells, ×105 (%within gated CD43+cells) |
1.9 ± 0.7 (15.9 ± 5.7) |
0.1 ± 0.0 (2.9 ± 0.5) |
0.7 ± 0.3 (10.8 ± 4.2) |
< 0.1 (1.3 ± 0.2) |
0.4 ± 0.1 (9.9 ± 1.1) |
0.3 ± 0.1 (11.9 ± 3.5) |
1.4 ± 0.4 (14.9 ± 2.1) |
1.5 ± 0.4 (11.8 ± 3.3) |
0.6 ± 0.3 (4.3 ± 1.7) |
0.8 ± 0.3 (8.3 ± 1.0) |
0.2 ± 0.1 (6.0 ± 2.6) |
0.9 ± 0.6 (4.2 ± 2.3) |
*
The absolute number of cells generated from one 10 cm dish of hESC/OP9 coculture is shown. The percentage of cells within indicated cell population is displayed in parentheses. Results are mean±SE of 3 to 5 independent experiments.