Table 5.

Timings for PK collection

Edotecarin PK	Pre-edotecarin infusion	During edotecarin infusion	Post-end edotecarin infusion
Cycle 1 (12 samples)	Within 30 min before start of infusion	15, 30 and 50 min after start of infusion	5, 15, 30 min and 1, 1.25, 1.5, 2, 3, 4, 6, 8, 24, 48, 72 and 96 h
Cycle 2 (10 samples)	Within 30 min before start of infusion	50min after start of infusion	5min and 1, 2, 4, 24, 48, and 72 h
5-FU PK	During 5-FU infusion		Post-end 5-FU infusion
Cycle 1 (5 samples)	3 h, 24 h, and 45 h+ 50 min, after start of infusion		10 min and 1 h
Cycle 2 (5 samples)	3 h, 24 h, and 45 h+ 50 min, after start of infusion		10 min and 1 h

5-FU, 5-fluorouracil; PK, pharmacokinetics.

Each blood sample was placed on ice immediately after collection and, within 60 min for edotecarin samples and 30 min for 5-FU samples, centrifuged for harvesting of plasma. Centrifugation was done either at room temperature or at 4°C. Plasma was stored frozen (at or below −20°C).