Table 1.

Catalytic Properties wild-type MT Enzyme and Mutants

Enzyme	Mutation	Malonyl CoA			Acetyl CoA
		KM (µM)	kcat (min−1)	kcat/ KM	KM (µM)	kcat (min−1)	kcat/ KM
MTa	WT	3.1 ± 0.6	65.1 ± 6.1	21	-	0	-
	R142Q	38.6 ±9.6	0.13 ± 0.01	0.003	9.1 ± 1.25	0.37 ± 0.03	0.04
	R142G	71.2 ± 21	0.17 ± 0.02	0.0023	32.2 ± 6.9	0.40 ± 0.05	0.012
	R142A	1060 ± 99	0.37 ± 0.02	0.00035	32.9 ± 5.7	0.29 ± 0.04	0.009
MATb	WT	1.9 ± 0.23	72 ± 0.5	37.9	3.9 ± 0.4±	114 ± 3.6	29.2
	R606A	16.2 ±2.4	0.6 ± 0.048	0.037	1.8 ± 0.28	852 ±12	473

^aAssayed at 20 °C.

^bAssayed on the rat enzyme at 0 °C; data from {Rangan, 1997 #7104}.