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. Author manuscript; available in PMC: 2011 Jul 29.
Published in final edited form as: Neuron. 2010 Jul 29;67(2):224–238. doi: 10.1016/j.neuron.2010.07.001

Figure 6. Recovery of CaV Currents after Dr-VSP-Induced Inhibition.

Figure 6

(A and E) Current traces for CaV1.3 (A) and CaV2.2 (E) channels in control (top) and Dr-VSP-expressing (bottom) cells before and after a +120-mV/1-s depolarizing pulse. CaV1.3 and CaV2.2 currents were measured at −10 mV and +10 mV, respectively, at the indicated times after the +120-mV pulse. Dashed lines indicate zero current, and dotted lines, the initial CaV current before the depolarization step.

(B and F) Time course of recovery of CaV1.3 and CaV2.2 currents after the Dr-VSP-induced inhibition in control (open circles) and Dr-VSP-expressing (closed circles) cells. Data are mean ± SEM (CaV1.3, n = 6 for both control and Dr-VSP; CaV2.2, n = 5 for both). Inset shows % current relative to control cells.

(C and G) Time course of CaV1.3 and CaV2.2 current recovery in cells transfected with PIPKIγ (CaV1.3, n = 5 for control and n = 8 for Dr-VSP; CaV2.2, n = 4 for control and n = 5 for Dr-VSP). Inset shows % current, comparing Dr-VSP to control cells.

(D and H) Time course of CaV1.3 and CaV2.2 current recovery with 3 mM AMP-PCP instead of ATP in the pipette solution. Data are mean ± SEM (CaV1.3, n = 7 for control and n = 10 for Dr-VSP; CaV2.2, n = 6 for control and n = 5 for Dr-VSP). Insets show the % current recovery in the presence of AMP-PCP. See also Figure S4.