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. 2001 Feb 6;98(4):1699–1704. doi: 10.1073/pnas.041593198

Figure 4.

Figure 4

Average AT contents of MITEs and their flanking sequences compared with STS and EST sequences in the A. gambiae database. AT contents of all full-length MITEs (see Table 1 for sample sizes) and their flanking sequences (STS minus MITE, indicated by the suffix “F”), and all of the 17,509 STS sequences in the A. gambiae genome database were calculated. Calculations of the Pegasus elements and their flanking regions were based on sequences reported by Besansky et al. (22). The forward and reverse sequences of the A. gambiae ESTs were analyzed separately because many of them represent pairs of sequences covering different regions of the same clone. Two hundred ESTs were randomly selected from each of the 2,990 forward ESTs and the 2,936 reverse ESTs (27). They were analyzed by using blast to remove redundancy that resulted from multiple copies of cDNAs. AT contents of 186 nonredundant forward ESTs (EST-For) and 181 nonredundant reverse ESTs (EST-Rev) were calculated and analyzed. Data points represent the mean AT contents. The error bar represents the SEM. Note that the standard errors for several data points are too small to be shown at the current scale. Mann–Whitney tests were used to compare the medians at α = 0.05. In most cases, t-tests were also used to compare the means, which gave the same conclusions. Samples in tier I have significantly higher AT contents than samples in tier II and III, whereas most samples in tier II have significantly higher AT contents than samples in tier III. One exception is TAA-I-AgF of tier II, which has a small sample size. Its AT content is neither significantly higher than samples in tier III nor significantly lower than TA-Iα-AgF, TA-IIα-AgF, TA-IV-AgF, and TA-IV-Ag of tier I. The other exception is the comparison between TAA-I-Ag and TA-IV-Ag, which is not significantly different. Samples in tier II are not significantly different from each other while EST-For is slightly more AT-rich than EST-Rev in tier III (P = 0.045). A few samples in tier I are slightly more AT-rich than others.