Figure 3.

Effect of oligomycin, which blocks the mitochondrial F₁F₀-ATPase, or respiratory inhibitors, cyanide or antimycin A, on membrane potential-dependent CMX labeling of eosinophil organelles. (A) Eos were cultured in medium with oligomycin at 0 μg/ml (closed circles), 0.1 μg/ml (open circles), 1.0 μg/ml (closed squares), 10 μg/ml (open squares), and 20 μg/ml (closed triangles) for 0, 0.5, 2, 7, and 9 h. Data indicate the percent of Eos retaining the CMX within the mitochondria of four separate Eos preparations ± SEM. (B) Eos were untreated (closed circle) or treated with 5 mM KCN (open circle) or 10 μg/ml antimycin A (closed square). CMX retention was measured after 0, 0.5, 2, 7, and 24 h from 3 separate Eos preparations ± SEM.