Fig. 7. Known Members of the Ptx and Otx Families of HD Proteins Do Not Comigrate with Complex 2.
EMSA was performed using the LH 121/87 probe and nuclear extracts from LβT2 cells, NIH3T3 cells, and NIH3T3 cells transiently transfected with an expression vector coding for Otx1 (3T3 + Otx1), Otx2 (3T3 + Otx2), Ptx1 (3T3 + Ptx1), or Ptx2a (3T3 + Ptx2a), as indicated above each lane. Open arrows at right indicate the migrations of the Otx and Ptx family members as labeled. The closed arrow at left indicates complex 2.