Fig. 2.

Activin and glucocorticoids do not synergistically activate MMTV, LHβ, or GnRHR gene expression. A–C, The MMTVluc reporter gene (A), the −1800LHβluc reporter gene (B), or the −1200Gn-RHRluc reporter gene (C) were transiently transfected into LβT2 cells along with 200 ng GR and TKβgal. After overnight starvation in serum-free media, the cells were treated for 24 h with vehicle (0.1% ethanol), 10 ng/ml activin, and/or with 100 nM dexamethasone (Dex) as indicated. *, Hormone treatment significantly different from the vehicle-treated control, using one-way ANOVA followed by Tukey's post hoc test. None of the cotreatments were synergistic by two-way ANOVA.