Fig. 6.

Cis mutations affecting activin responsiveness also reduce synergy. The wild-type −1000FSHβluc reporter gene or one of six mutated reporters was transiently transfected into LβT2 cells along with TKβgal and GR. After overnight starvation in serum-free media, the cells were treated for 24 h with vehicle (white bars) or with either 100 nm dexamethasone (Dex, black bars) (A) or 10 ng/ml activin (Act) and 100 nm dexamethasone (Dex, black bars) (B). *, Hormone treatment significantly different from the vehicle-treated control of the same reporter gene by Student's t test; #, induction of the mutant reporter gene is significantly reduced from the induction of the wild-type reporter gene, using one-way ANOVA followed by Tukey's post hoc test.