Fig. 8.

Smad3 and GR are sufficient to induce synergy. The wild-type −1000FSHβluc reporter gene, GR, and TKβgal were transiently transfected into LβT2 cells along with either empty vector or Smad3 as indicated. After overnight starvation in serum-free media, the cells were treated for 24 h with either vehicle or 100 nm dexamethasone (Dex). *, Hormone treatment significantly different from the vehicle-treated control, using one-way ANOVA followed by Tukey's post hoc test; †, synergy between activin and dexamethasone by two-way ANOVA.