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. Author manuscript; available in PMC: 2010 Sep 2.
Published in final edited form as: J Biol Chem. 2005 Mar 1;280(19):19156–19165. doi: 10.1074/jbc.M502004200

Fig. 3. MSX and DLX family members bind to the homeodo-main sites.

Fig. 3

A, EMSA analysis and antibody supershifts were performed using GT1-7 nuclear extract as well as antibodies recognizing MSX1, and DLX2 (lanes 3 and 4) and an IgG control (lane 5). The oligonucleotide probe corresponds to −1642/−1623 of the rat GnRH gene and contains the 5′ most homeodomain-binding site in the enhancer. The thick arrow marks the major complex and the thin arrow marks the minor complex. An arrowhead indicates the supershift of the minor complex. B, EMSA analysis and antibody supershifts were performed using the same oligonucleotide probe, NLT nuclear extract and an antibody recognizing DLX1 or MSX2 (lanes 2 and 3) or an IgG control (lane 4). The thick arrow represents the major complex and the thin arrow represents the minor complex. N.S. represents nonspecific binding, and F.P. indicates excess, free probe.