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. Author manuscript; available in PMC: 2010 Sep 2.
Published in final edited form as: J Biol Chem. 2005 Mar 1;280(19):19156–19165. doi: 10.1074/jbc.M502004200

Fig. 7. GnRH-positive cells in the Dlx1&2 or Msx1-null mouse embryos.

Fig. 7

In situ hybridization of Dlx1&2 or Msx1-positive and null embryonic mice ages 12.5, 13.5, and 16.5 dpc was carried out as described using a DIG-GnRH probe. Images represent parasagittal sections of the wild-type (left panel) and null (right panel) embryos from 13.5-dpc Dlx1&2 (panel A), 16.5-dpc Dlx1&2 (panel B), 12.5-dpc Msx1 (panel C), 13.5-dpc Msx1 (panel D), and 16.5-dpc Msx1 (panel E). Panel F, 13.5-dpc Msx1-null embryo with GnRH-positive cells in the olfactory epithelium (arrow) at low (left panel) and higher (right panel) magnification are indicated. Panel G, immunohistochemical analysis of GnRH-positive cells located in the olfactory epithelium of Msx1-null embryos at 13.5 dpc. GnRH-positive cells located in the tectum of 13.5-dpc Msx1-null (panel H), 16.5-dpc Dlx1&2-null (panel I), 16.5-dpc wild-type (panel J), and 16.5-dpc Msx1-null (panel K) embryo. Arrows indicate GnRH-positive cells located outside of the characterized migratory route for the septohypothalamic population. Black scale bars represent 100 μm in the lower left of each panel. The schematic represents a parasagittal view of a mouse embryo head in the region where GnRH-positive neurons are found.