Table I. GnRH-positive cells counted by in situ hybridization of mouse embryos.
In situ hybridization was performed using an antisense RNA probe for GnRH to assess the number of GnRH-positive cells. Whole heads of mouse embryo littermates were sectioned, hybridized, and then counted for GnRH-positive cells.
| Genotype | Embryo age | Nose | Primordium of the septum | Tectum | Total (septohypothalamic) | |
|---|---|---|---|---|---|---|
| dpc | ||||||
| Dlx1&2 | +/+ | 13.5 | 668 ± 74 | 277 ± 92 | 0 | 945 ± 62 |
| Dlx1&2 | −/− | 13.5 | 515 ± 125 | 97 ± 60 | 0 | 612 ± 177 |
| Dlx1&2 | +/+ | 16.5 | 153 ± 40 | 833 ± 34 | 203 ± 29 | 985 ± 16 |
| Dlx1&2 | −/− | 16.5 | 72 ± 36 | 610 ± 45 | 204 ± 18 | 681 ± 81 |
| Msx1 | +/+ | 12.5 | 733 ± 15 | 125 ± 3 | 0 | 858 ± 18 |
| Msx1 | −/− | 12.5 | 896 ± 52 | 137 ± 14 | 0 | 1,032 ± 54 |
| Msx1 | +/+ | 13.5 | 710 ± 44 | 229 ± 31 | 0 | 939 ± 20 |
| Msx1 | −/− | 13.5 | 1,021 ± 99 | 267 ± 25 | 19 ± 6 | 1,300 ± 116 |
| Msx1 | +/+ | 16.5 | 309 ± 58 | 728 ± 63 | 238 ± 26 | 1,037 ± 103 |
| Msx1 | −/− | 16.5 | 227 ± 49 | 643 ± 64 | 215 ± 31 | 871 ± 53 |