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Competition assays with an AP-1 consensus oligonucleotide. Radioactive labeled probe (3 fm) and 10-, 50-, 100-, or 200-fold molar excess of unlabeled competitor were added simultaneously to 2 μ g nuclear extract from LβT4 cells obtained as described in Materials and Methods. The competitors were the unlabeled probe (−163), unlabeled probe with a mutation in the putative AP-1 site (−163M), and an AP-1 consensus oligonucleotide.
