Table 1. Experimental design summary.
Experiments performed | |||||
---|---|---|---|---|---|
Experiment |
Effect of mapracorat on hyperosmolar-induced cytokine release |
Effect of mapracorat on hyperosmolar-induced cytokine release |
Effect of RU-486 on mapracorat inhibition of hyperosmolar-induced cytokine release |
Effect of mapracorat on hyperosmolar-induced MAPK phosphorylation |
Effect of mapracorat on hyperosmolar -induced AP-1 and NFκB activities |
Technique |
Multiplex Luminex |
Multiplex Luminex |
Multiplex Luminex |
Cell-based ELISA |
Reporter gene assay |
Cell type |
T-HCEpiC |
P-HCEpiC |
T-HCEpiC |
T-HCEpiC |
T-HCEpiC |
Dose range of mapracorat and dexamethasone | 1–1000 nM | 1–1000 nM | 1–1000 nM | 0.1–10 µM | 0.1–1 µM |