TABLE 2 .

List of biotinylated peptides identified in InvG within the needle complex^a

Exptl mass	Charge state	Peptides identified	Denatured NC	Native NC	Labeled residue(s)
645.336	3	NVSLNEFNNFLKR	+	+	Lys-127
702.69	3	SGLYNKNYPLR	+	+	Lys-134
847.091	3	QSGAWSGDDKLQKWVR	+	+	Lys-545, Lys-548
1,020.498	3	KGTFYVSGPPVYVDMWNAATMMDK	+	+	Lys-144, Lys-168
661.027	3	DQKMVIPGIATAIER	+		Lys-201
778.42	2	EPVIVSKMAAR	+		Lys-61
492.762	2	GQEAIK	+		Lys-562
815.937	2	VYLDRGQEAIK	+		Lys-562
1,001.027	2	IPVTGSGFVAKDDSLR	+		Lys-38
1,048.889	3	TFFDAMALQLK EPVIVSKMAAR	+		Lys-54, Lys-61
783.921	2	TFYTKLIGER	+		Lys-392
585.827	2	QKIGVMR		+	Lys-179
729.723	3	QKIGVMRLNNTFVGDR		+	Lys-179

^aPurified NCs were treated with a lysine specific biotin reagent directly (native NC) or after denaturation in SDS (denatured NC) prior to preparation for LTQ-Orbitrap mass spectrometry. The peptides identified were ±5 ppm in terms of mass accuracy.