FIG 2 .

Accumulation of glycated proteins and AGEs following Gcp depletion. Lysates were extracted from wild-type and Gcp-depleted cells and further separated into proteins and low-molecular-mass compound fractions, as described in Materials and Methods. (A) Proteins were subjected to SDS-PAGE, followed by staining with Coomassie brilliant blue for total proteins (c) or diol-specific silver stain for glycated proteins (s). (B) Quantification of glycated proteins by use of a periodate-based colorimetric assay. (C) AGE-specific fluorescence (relative; excitation at 370 nm and emission at 440 nm) following Gcp depletion in total lysates and in the fractions of purified proteins and low-molecular-weight (MW) compounds. The results represent three independent experiments.