FIG. 3.

Average sequence variability of V3 loop positions mapped onto the 3D structure of the 2219 mAb-bound conformation of the crown of the V3 loop of clade B strain RP322 consisting of residues 8–23 of the V3 loop ⁸KRKRIHIGPGRAFYTT²³. The V3 loop crown region is divided into four different zones: the left-most division (orange) contains the base of the V3 crown (⁸KRK¹⁰ and ²³T). The upper middle division (red) contains the residues pointing their side chains into the hydrophilic face of the amphipathic ß-hairpin (¹¹R, ¹³H, ¹⁹A and ²²T). The lower middle division (blue) contains residues that point their side chains into the hydrophobic face of the amphipathic ß-hairpin (¹²I, ¹⁴I, ²⁰F and ²¹Y). The division on the right (green) contains the conserved ß-turn (¹⁵GPGR¹⁸). The average sequence variability score, calculated as noted in Materials and Methods, is noted in each region.