FIG. 4.

Cytokines detected in the lungs of vaccinated and unvaccinated perforin KO and wild type animals. Perforin-KO and wild type (B6) mice were vaccinated with DNA (D) and vaccinia virus (V) in a prime-boost regimen. Inoculations were in the order D-D-D-V. Two months after the last injection, vaccinated and unvaccinated mice were challenged with rSeV-env. Mice were given ip injections with the anti-CD8 2.43.1 mAb on days −5, −3, −1, +1, and +3 relative to rSeV-env challenge. BAL fluid from vaccinated and unvaccinated mice was examined for IL-2, INF-γ, IL-4, and IL-5 on day 5 after challenge. Results are shown for individual B6 (top) and perforin-KO (bottom) mice.