Fig. 1.

Expression of EMT marker genes in breast cancer cell lines and nontransformed EMT-induced human mammary epithelial cells. (A) Expression of EMT marker genes was measured by semiquantitative RT-PCR performed on RNA extracted from MCF-7, MDA-MB 231, MDA-MB 435, and SUM1315 cells. (B–D) HMLE cells were transduced with a retrovirus overexpressing the indicated gene and expression of the indicated gene was measured by semiquantitative RT-PCR (B) or quantitative PCR (C and D). GAPDH (C) or U6 snoRNA (D) was amplified for normalization. Error bars represent the SD from three independent experiments.