Skip to main content
. 2010 Aug 16;107(35):15491–15496. doi: 10.1073/pnas.0914785107

Fig. 3.

Fig. 3.

miR-204 is required for establishment of D-V polarity of the optic cup. Frontal vibratome sections of control (A–C), Mo-miR-204 (D–F), miR-204 (G–I), Mo-Meis2/Mo-miR-204 (J–L), Meis2-TPmiR-204 (M–O), and Meis2-TPmiR-204/miR-204 (P–R)–injected embryos processed for whole-mount RNA ISH with probes specific for olPax6 (A, D, G, J, M, and P), olVax2 (B, E, H, K, N, and Q), and olTbx5 (C, F, I, L, O, and R). olPax6 is up-regulated and ectopically expressed in ventral retina of morphant embryos (D and M, asterisk). Expression of ventral gene olVax2 is reduced, whereas that of dorsal marker olTbx5 is expanded ventrally in morphant embryos (E, F, N, and O) when compared with control embryos (B and C). miR-204–overexpressing embryos show reciprocal alterations in Pax6 and D-V marker expression (G–I). Defects in D-V optic cup polarity are completely rescued by coinjection of Mo-miR-204 with Mo-Meis2 (J–L) and Meis2-TPmiR-204 with miR-204 (P–R). (Scale bars: 20 μm.)