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(A) Coimmunoprecipitation of p110α with WT p85 and with p85 mutants. (Upper) p110α is included in an immunoprecipitate of WT and of mutant p85. (Lower) Graphs show that under the conditions of the pull-down, the two mutants still bind efficiently to p110α. (B) Coimmunoprecipitation of p110β with WT p85 and with p85 mutants. Upper and Lower as in A.
