Fig. 3.

Measurement of increased ploidy by FISH. (A) Example of shRNAs that increase ploidy as measured by FISH. NL-20 cells were infected with the indicated kinase shRNAs and fixed for FISH analysis after 4 d. A centromeric probe specific for chromosome 12 (red) was hybridized to nuclei, which were then counterstained with DAPI (blue). sh, short hairpin. (B) Quantitation of FISH analysis for chromosome 12. FISH analysis was carried out with the indicated shRNAs in NL-20 and HBE135 cells. The number of nuclei with greater than two copies of chromosome 12 for each shRNA was measured using CellProfiler software. For each shRNA, at least 50 nuclei were counted.