Fig. 4.

Knockdown of several kinases results in multipolar mitoses and aneuploidy. (A) Knockdown of kinases results in multipolar mitoses. Kinase shRNAs were transduced into HBE135 cells, and the cells were selected for 21 d. Cells were fixed for immunofluorescence and stained with antibodies against tubulin (green) and pericentrin (red). DNA was stained with DAPI (blue). (Left) Examples of multipolar mitosis phenotypes are shown. (Right) Graph shows the mean number of poles for each sample (n = 25 for each). *P < 0.05 by Student's t test. (B) Sustained knockdown of NME1 results in a progression from tetraploidy to aneuploidy. HBE135 cells were transduced with the NME1 shRNA and selected for 4 or 17 d. Cells were fixed for FISH analysis and stained with centromeric probes for chromosomes 8 and 12. The number of copies of these two chromosomes in each nucleus is shown. (Right) Number of copies of chromosome 8 in nuclei with four copies of chromosome 12.