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. 2010 Aug 17;107(35):15467–15472. doi: 10.1073/pnas.1000462107

Fig. 4.

Fig. 4.

PKA-dependent HDAC5 phosphorylation and nuclear retention repress MEF2-dependent gene transcription and cardiac fetal gene expression. (A–C) Luciferase reporter assays for MEF2 transcriptional activity. NRVMs were infected with adenoviruses expressing 3xMEF2-luciferase reporter gene (A) along with adenoviruses expressing Flag-HDAC5-WT or Flag-HDAC5-S280A (B) or Flag-HDAC5-S280D (C) and then were pretreated with forskolin or cAMP for 30 min, followed by stimulation with PE for 24 h. *P < 0.05 compared with PE + vehicle; n = 4. (D and E) RT-PCR analysis of cardiomyocyte fetal gene expression. NRVMs were infected with adenoviruses expressing GFP alone (control), GFP-HDAC5-WT, or GFP-HDAC5-S280A for 24 h and then were pretreated with cAMP, followed by stimulation with PE for 24 h. The mRNA was extracted from the cell lysates; then RT-PCR with the primers for ANF, β-MHC, α-SMA, and GADPH (internal control) was performed. *P < 0.05 versus PE + Ad-GFP; #P < 0.05 versus PE + GFP-HDAC5-WT; n = 4.