Fig. 3.
Recognition of Aβcc oligomers by antibodies that are conformation specific for wild-type Aβ aggregates. (A) mAb158 antibody sandwich ELISA detection (22) of Aβ42cc β-sheet oligomers/protofibrils compared with detection of wild-type Aβ42 protofibrils. (B) mAb158 ELISA analysis of different Aβ42cc species at 83 pM total peptide concentrations. (C) Aging of Aβ42cc LMW and β-sheet oligomers at 37 °C followed by A11 antibody dot blot analysis showing that A11 binding aggregates form from LMW oligomers but not from β-sheet oligomers. Concentrations were equalized before blotting.