Fig. 4.

SEX4 possesses a contiguous ligand-binding active site. (A) Surface view of the DSP active site to CBM binding site pocket. F167 (yellow), S198 (red), and W278 (green) are all highlighted. (B) SEX4 specific phosphatase activity expressed as the ratio of glucan phosphatase activity/generic p-NPP activity. Glucan phosphatase activity was quantified as pmol min^-1 mg^-1 of phosphate release. The activity against p-NPP was quantified as μmol min^-1 mg^-1. Δ89-SEX4 (WT), C198S, and three F167 mutants were analyzed. Error bars indicate means ± the standard deviation.