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. Author manuscript; available in PMC: 2011 Aug 27.
Published in final edited form as: Chem Biol. 2010 Aug 27;17(8):841–851. doi: 10.1016/j.chembiol.2010.05.026

Figure 7. Stopped-flow analysis of PimD interactions with iodosobenzene and peroxynitrite.

Figure 7

A, Rapid-scan absorbance spectra for the first 45 s of reaction between PimD (5 μM) and iodosobenzene (150 μM) selected in 3 s intervals are shown. Inset: kinetics recorded at 435 and 380 nm. B, singular value decomposition analysis of data in A using A→ B → C → D kinetic model, with k1 = 0.271 s−1, k2 = 1.141 s−1, and k3 = 0.0581 s−1. Inset: time dependence of the different forms of PimD in the reaction with iodosobenzene. C, rapid-scan absorbance spectra for the first 45 s of reaction between PimD (5 μM) and peroxyntrite (250 μM) selected in 3 s intervals are shown. Inset: kinetics recorded at 433 nm. All data were collected at 10°C in 100 mM potassium phosphate, pH 7.4.