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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Comp Neurol. 2010 Oct 1;518(19):3948–3961. doi: 10.1002/cne.22432

Figure 6.

Figure 6

Simultaneous localization of TRH with oxytocin (OT) and vasopressin (AVP) in the PVN of mice. Medium-magnification confocal images illustrate the distribution of the TRH-(red), AVP- (blue, A1), OT- (blue, B2) and Fluoro-Gold-IR (green) cells at the mid level of the PVN. A1-2 or B1-2 images represents the same field. Note that TRH is not colocalized with the magnocellular peptides. Arrows point to single-labeled TRH neurons, while arrowheads label the hypophysiotropic TRH neurons. Each image represents 1 μm thick optical slices. III, third ventricle, Scale bar = 50 μm.