Skip to main content
. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Nat Neurosci. 2010 Aug 22;13(9):1059–1065. doi: 10.1038/nn.2618

Figure 6.

Figure 6

Molecular mechanism of action of Pias3 in developing cones. (a) Quantitative sequential ChIP analysis for Trβ2 and Pias3. (b) Quantitative sequential ChIP analysis for Trβ2 and Sumo1. (c and d) Pias3-dependent SUMOylation activates expression of M-cone opsin luciferase reporter constructs in the presence of either Crx or Trβ1 (c) or Trβ2 (d). SUMOylation-deficient mutants (Pias3 ΔSUMO) could not enhance reporter expression. (e and f) Rorα activates expression of S-cone opsin luciferase reporter constructs in the presence of Crx. Rorα represses expression of S-cone opsin luciferase reporter constructs in the presence of Crx, Trβ2 and Rxrγ, and Pias3-dependent SUMOylation of Rorα enhances this repression (e). SUMOylation site mutants of Rorα (K185R) showed less efficient repression (f). ‘+’ and ‘++’ in Fig. 6c-f represent 10 ng and 20 ng, respectively. All data in Fig. 6c-f are represented as mean ± s.d. (n = 3). * p < 0.05 (Student’s t-test).