Table 2. Averaged characteristics of cardiac defects following temperature shock.
| Temperature Shock | N | Pre-stimulus Frequency (BPM) | Post-stimulus Frequency (BPM) | (Pre-Post) Frequency Variation (%) | Pause Duration (sec/5 min) | Delay of Basal Frequency Recovery (sec) | |
| 36°C | WT | 11 | 152.4±2.7 | 173.8±2.7 | +14.1 | no pause | - |
| pain3 | 5 | 194.4+/+5.6 | 207.2±3.9 | +6.6ns | no pause | - | |
| pain4 | 5 | 198.4±3.9 | 213.6±5.3 | +7.7ns | no pause | - | |
| RNAi 39477 | 11 | 183.6±2.3 | 200.7±2.3 | +9.3ns | no pause | - | |
| 1029gal4×pain3 | 10 | 148.8±2.4 | 166.8±2.3 | +12.2ns/ns | no pause | - | |
| 46°C | WT | 15 | 148.3±2.9 | 113.1±4.2 | −23.7 | no pause | - |
| pain3 | 7 | 200.6±3.1 | 241.1±4.1 | +20.2*** | no pause | - | |
| Pain4 | 5 | 202.4±8.2 | 244.8±6.4 | +20*** | no pause | - | |
| RNAi 39477 | 5 | 182.4±4.1 | 215.2±2.9 | +17.9*** | no pause | - | |
| RNAi 39478 | 5 | 188±4 | 216.8±6.6 | +15.3*** | no pause | - | |
| 1029gal4×pain3 | 10 | 148.6±2.4 | 121.2±4.3 | −18.5ns/*** | no pause | - | |
| 50°C | WT | 10 | 152.8±2.2 | arrest | - | 26.8±2 | 43.8±2.4 |
| pain3 | 6 | 193.3±2.2 | 243.3±8 | +25.9 | no pause | - | |
| Pain4 | 5 | 202±2.7 | 257.6±5.3 | +27.3 | no pause | - | |
| RNAi 39477 | 5 | 183.2±2.3 | fibrillation | - | no pause | - | |
| RNAi 39478 | 6 | 178.7±6 | fibrillation | - | no pause | - | |
| 1029gal4×pain3 | 10 | 146±3.1 | arrest | - | 25.5±3ns | 42.5±2.3ns | |
Frequencies, pauses and number of pauses are expressed as mean ± SD. The pause duration (in sec) corresponds to the cumulative time of pauses during 5 min. All experiments are performed in Schneider medium. The number of experiments is indicated in the Table. For 1029Gal4×pain3, statistical significance is indicated on the left for data compared to wild-type and on the right when they are compared to pain3. In RNAi lines, observed phenotypes are weakly than in mutants probably because of a lower efficiency of RNAi in inactivating Painless. **p<0.0005; ***p<0.0001; n.s.: non significative.