Figure 3. Phenotypes associated with mir-57 loss of function and misexpression.

Homozygous mir-57(gk175) animals placed at 26°C occasionally had preanal bulges (arrowhead in (A)) or vacuolated cells (arrow in (B)) in the tail. The animal in (B) also exhibited a forked tail spike (arrowhead). mir-57 overexpression from extrachromosomal arrays resulted in Nob (C,D) and Vab-like (E) tail phenotypes as indicated by arrowheads. A wild-type larva (F) is shown for comparison. Males carrying the mir-57 extrachromosomal arrays produced abnormal tails (G) compared to wild type (H). The anus is indicated by arrowheads and tail rays by arrows. Scale bars indicate 20 µm in all pictures.