Table 2. Phenotypes of mir-57 transgenic arrays.
Promoter | miRNA | Position | Lines | Vab/Nob% | Background |
mir-57 | mir-57 | −2260 to 234 b | 8 | 29.3(625 d) | wild type |
mir-57 | none | −2260 to −63 b | 5 | 33.9(373) | wild type |
mir-57 | mir-57 | −2260 to 234 b | 2 | 36.4(522) | mir-57(gk175) |
mir-57 | none | −2260 to −63 b | 6 | 0(583) | mir-57(gk175) |
vab-7 | mir-57 | −3664 to 36 c | 3 | 11.3(362) | wild type |
vab-7 | mir-57m a | −3664 to 36 c | 7 | 0(347) | wild type |
vab-7 | none | −3664 to 36 c | 5 | 0(831) | wild type |
aMutated mir-57 mature sequence (see Materials and Methods).
bCoordinates in base pairs relative to the start of the mature mir-57 sequence.
cCoordinates in base pairs relative to the translational start of vab-7 sequence. The sequences are fused with mir-57 genomic region (−50 to 234 bp relative to the start of mature mir-57 sequence).
dNumber of animals scored.