Figure 2. T3 induces the transcription of the L/M opsin gene in WERI cells.

WERI cells were either treated with vehicle (mock), 10 uM all-trans retinoic acid (ATRA), 10 uM 9-cis retinoic acid (9c-RA), or 10 nM T3 (T3) for 48 hours. RNA extracted from these samples was used to perform RT-PCR analyses using primer pairs that amplify the L/M opsin gene (top panel), COUPTF1 (middle panel), or the housekeeping gene GAPDH (bottom panel).