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A*TPγS analog orthogonality and acceptance by AS kinases. (a) Chemical structure of A*TPγS, R indicates the site of N6 modification. (b) Kinase reactions with ATPγS and A*TPγS analogs. Following PNBM alkylation, the labeled substrates were detected with α-hapten-IgG western blot analysis, full-length blots are available in Supplementary Figure 1d. WT, wild type.
