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(A) Protofibrils induced increase of membrane conductivity. Buffer, 1 µM native Ure2, 1 µM fibrils and 1 µM protofibrils of Ure2 were added sequentially. (B) Protofibrils increased the membrane conductivity in a concentration dependent manner. Currents were recorded in response to voltage ramps from −100 to +100 mV at a rate of 50 mV/s. Curves are labeled with the concentration of Ure2 protofibrils used in the assay.
