Figure 6. Supplementation by exogenous amino-oxyacetate in medium cannot induce erythroid differentiation but does induce cell death.

A. Cells were treated with different concentrations of amino-oxyacetate and expression levels of the erythroid marker glycophorin A on the surface of K562 cells were assessed using a mouse FITC-conjugated anti-human glycophorin A antibody. Each histogram is representative of three experiments. B. K562 cells with or without ME2 knockdown were incubated with different concentrations of AOA for 72 h. Cell death was assessed by flow cytometry. Top: pLKO; Bottom: shME2-3. Data are representative of two independent experiments.