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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: J Infect Dis. 2010 Aug 15;202(4):624–632. doi: 10.1086/654929

Figure 2.

Figure 2

Synergy of hemoglobin (Hb) with killed Escherichia coli and Staphylococcus aureus. Bone marrow–derived macrophages from C57BL/6 mice were washed 3 times with serum-free medium and then cultured overnight (A, B, E, and F ) or at different times (C, D, G, and H ) with heat-killed E. coli at indicated concentrations (A and B ) or at 105 cfu/mL (C and D ) or with S. aureus at indicated concentrations (E and F ) or at 107 cfu/mL (G and H ) in the absence or presence of Hb at 300 μg/mL. Concentrations of tumor necrosis factor (TNF) (A, C, E, and G ) and interleukin (IL)–6 (B, D, F, and H ) in the supernatants were determined by enzyme-linked immunosorbent assay. The results denote the mean ± standard error and are representative of 4 independent experiments. *P <.05, **P <.01, ***P <.001, compared between cells treated with and without Hb.