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. Author manuscript; available in PMC: 2010 Sep 2.
Published in final edited form as: Stem Cells. 2008 Jul 31;26(10):2516–2522. doi: 10.1634/stemcells.2008-0379

Figure 3.

Figure 3

JK1 cells support spermatogonial progenitor cell (SPC) proliferation without loss of germ cell markers. (A): Bright-field images (original magnification, ×100) of a large SPC colony on JK1 and (A inset) mouse testis stroma (MTS) demonstrated similarities in size and shape of SPC colonies grown on these two feeders. (B–D): Immunohistochemistry for (B) PLZF, (C) GCNA, and (D) DAZL revealed that the expression of these spermatogonial stem cell markers was maintained in SPCs cultured on JK1 cells for multiple passages. Antibody staining is red; nuclear counterstain is blue. All size bars = 50 μm.