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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cell Death Differ. 2010 May 28;17(12):1867–1881. doi: 10.1038/cdd.2010.53

Figure 3.

Figure 3

Figure 3

Figure 3

Figure 3

Figure 3

As2O3 induces autophagy in ovarian cells. (a) T80 (left panel), HEY (middle panel), and SKOV3 (right panel) cells were seeded at 250,000 cells per well in 6-well plates. After overnight attachment, the cells were treated with varying concentrations of As2O3 (2–50µM). After 18 hour incubation, the cell lysates were harvested and western analysis was performed using the following antibodies: (1) SnoN, (2) PARP, (3) Procaspase-3, (4) LC3, (5) p62, and (6) GAPDH as a loading control. The data shown are representative of 3 independent experiments. (b) HEY cells were plated at 250,000 cells per well in 6-well plate. After overnight attachment, cells were treated with 25µM As2O3 for varying times (0, 3, 9, 18, 24, and 30 hours) and light microscope images were captured at 40× magnification. The data shown are representative images. (c) HEY cells were seeded at 250,000 cells per well. Following overnight attachment, the cells were treated with 25µM As2O3 at various time points (3, 9, 18, 24, 30h). Cell lysates were harvested and western analysis was performed using the following antibodies: (1) EVI1, (2) SnoN, (3) TAK1, (4) TGFβRII, (5) SMAD2/3, (6) PARP, (7) Procaspase-3, (8) LC3, (9) Beclin-1, (10) p62, and (11) GAPDH as a loading control. The data shown are representative of 2 independent experiments. (d) and (e), Confluent HEY cells grown in T-75 flasks were treated with 25µM As2O3 for 3, 9, 18 hours and prepared for TEM. Images were captured at varying magnifications as indicated. Representative images are shown.